Website: Miller's Research Group
B.S. 1989, The University of Wisconsin-Madison; Ph.D. 1998, The University of Wisconsin-Madison; Postdoctoral/Associate Research Scientist 2001-2006, Columbia University
The Miller laboratory develops and applies chemical methods to label proteins and study their function in vitro and in living mammalian cells. With chemical labeling, a protein of interest is expressed as a fusion to a polypeptide tag that interacts specifically with a synthetic fluorophore or other chemical probe. Our experimental approach incorporates synthetic organic chemistry, molecular and cell biology, and microscopy. Specific projects include:
Development of protein-targeted lanthanide luminophores.
Lanthanide-based resonance energy transfer (LRET) imaging of protein-protein interactions in live cells.
Time-gated luminescence microscopy.
LRET-based high-thoughput screening of protein interactions and their inhibition.
Cell penetrating peptide (CPP)-mediated delivery of imaging probes.
Office: 5205 SES, MC 111