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Photo of Spille, Jan

Jan Spille

Assistant Professor

Physics and Chemistry


Building & Room:

SES 3136

Office Phone:

(312) 996-4588


Professor Spille’s research uses state-of-the-art fluorescence microscopy to resolve biophysical principles of structure and function in the cell nucleus. His lab works at the interface of Biology, Chemistry, and Physics. Current efforts in the lab are directed towards understanding how biomolecular condensates form and regulate processes such as transcription. For this purpose, the lab develops new tools for quantitative super resolution imaging of DNA, RNA, and proteins; and combines them with quantitative live cell imaging, cell line engineering, in vitro reconstitution, and computational modeling.

Selected Publications

Mediator and RNA polymerase II clusters associate in transcription-dependent condensates. WK Cho*, JH Spille*, M Hecht, C Lee, C Li, V Grube, II Cisse. Science, eaar4199 (2018). *contributed equallyy [Link]

Pol II phosphorylation regulates a switch between transcriptional and splicing condensates. YE Guo, JC Manteiga, JE Henninger, BR Sabari, A Dall’Agnese, NM Hannett, JH Spille, LK Afeyan, AV Zamudio, K Shrinivas, BJ Abraham, A Boija, TM Decker, JK Rimel, CB Fant, TI Lee, II Cisse, PA Sharp, DJ Taatjes, RA Young . Nature 572, 543-548 (2019).  [Link]

Direct observation of mobility state transitions in RNA trajectories by sensitive single molecule feedback tracking. JH Spille, TP Kaminski, K Scherer, JS Rinne, A Heckel, U Kubitscheck. Nucleic Acids Research 43 (2), e14 (2014). [Link]

A CRISPR/Cas9 platform for MS2-labelling of single mRNA in live stem cells. JH Spille, M Hecht, V Grube, W Cho, C Lee, II Cissé. Methods 153, 35-45 (2019). [Link]


BS, Physics (2007), Julius-Maximilians University Wuerzburg, Germany
MS, Physics (2009), Julius-Maximilians University Wuerzburg, Germany
PhD, Chemistry (2014), Rheinische Friedrich-Wilhelms University Bonn, Germany